This reduction in IFN responsiveness to repetitive stimulation—a process known as tachyphylaxis—was limited to STING, as transfection with Poly:IC after the fifth cGAMP stimulation led to an acute and robust ISG induction (Extended Data Fig. 10a), mirroring observations derived from cancer cells (Extended Data Fig. 8a). The gene discussed is IFNA1; the disease is cancer.