In the training cohort, univariable regression was used to identify fifteen significant risk factors, including Systolic Blood Pressure (SBP), Diastolic Blood Pressure (DBP), Homocysteine (HCY), Myoglobin (MB), C-reactive protein (CRP), Neuron-Specific Enolase (NSE), S100β, treatment history (thrombolysis, thrombectomy, antiplatelet, anticoagulation, statin, PPI), and complicates (dysphagia and stroke-associated pneumonia) (Table 2). The gene discussed is S100B; the disease is stroke disorder.