We used CRISPR to abolish the expression of Trim33 in a murine liver cancer cell line (p19/Nras), derived from an autochthonous tumor induced by Nras expression in the p19/Arf-null mice, which provides a tractable genetic model for in vitro and in vivo analysis (Fig. 1a)23. This evidence concerns the gene CDKN2A and neoplasm.