GSDMD and neoplasm: Interestingly, we found that ACS was released and vimentin was cleaved outside of larger GSDMD-N membrane holes after LGSN knockdown, indicating that a genetic absence of LGSN may permit chemo-drug influx through GSDMD pores by synergizing with a reduced dosage of 5-FU and L-OHP, leading to dramatic antitumor effects and significant xenotransplanted tumor regression.