Four mesenchymal markers (twist, snail, N-cadherin, and vimentin) were downregulated, whereas the epithelial marker (E-cadherin) was upregulated in TMEM26 RNAi KYSE270 and TE8 cells (Fig. 3D), indicating the reversed EMT process in TMEM26-depleting ESCC cells. This evidence concerns the gene TWIST1 and esophageal squamous cell carcinoma.