SLC31A1 and central nervous system cancer: We used 2 × RealStar Green Fast Mixture qPCR kit (GenStar, Cat: A301-101) to analyze the expression of the SLC31A1 gene in different glioma cell lines, in which GAPDH was used as the housekeeping gene, and its reliability was determined by the computational program RefFinder (Xie et al. 2023).