To further validate the co-cultured organoid model’s pathophysiological characteristics, we compared the expression of CGRP, Chromogranin A, NCAM and Synaptophysin in the patient SCLC tumor sections (Figure 2A), cell-culture derived xenografts (CDX) tumors (Figure 2B), 2D SCLC cell line cultures (Figure 2C) and in the developed organoid model (Figure 2D) at 14 days of culture. This evidence concerns the gene CHGA and neoplasm.