The outcomes disclosed that 8 out of 14 methylation sites in ccRCC had obviously negative correlation with ACSL1 expression, including cg03498175(r=-0.44, P < 0.001), cg08823975(r=-0.45, P < 0.001), cg24277788(r=-0.44, P < 0.001), cg01899937(r=-0.39, P < 0.001) and cg111668687(r=-0.37, P < 0.001) (Fig. 3C). The gene discussed is ACSL1; the disease is nonpapillary renal cell carcinoma.