In these studies, we added AF647-anti-CD31 to the slices to image vasculature in addition to PE-CD90.2 to image stromal cells in slices from mCerulean+ 4662 tumor-bearing mice at baseline (prior to FAP-CAR T cell administration) and 6, 12, and 18 hours post FAP-CAR T cell administration. The gene discussed is FAP; the disease is neoplasm.