Recent studies showed that the expression of chaperone proteins HSP10 and HSP60 and glutathione (GSH) was reduced in mitochondria due to treatment of APAP at toxic doses in mice that had been fasted overnight.234 The potential therapeutic benefits of GSH, HSP10 and HSP60 have been described in liver disease.235 A mechanism explaining the decrease in GSH content during liver disease first involves a decrease in the GSH biosynthesis rate. This evidence concerns the gene HSPE1 and liver disorder.