In the current study, in addition to fundamental expression control of TUG1 by MYC (Supplementary Fig. 1h), we found the ATR-CHK1-E2F pathway directly upregulated TUG1 expression in response to HU and CPT exposure (i.e. two hours of treatment), which induced more abundant RS and R-loop, in cancer cells. The gene discussed is TUG1; the disease is cancer.