TUG1 and glioblastoma: Because LN229 has a high level of DNA methylation in the promoter of MGMT (82.7% by pyrosequencing analysis), we examined the effect of TUG1 ASO and TMZ in another glioblastoma cell line U251MG, which has a lower level (23.2%) of DNA methylation in the MGMT promoter (Supplementary Fig. 10f).