Through our study, we demonstrated that TCF3 modulated the stemness of ESCC by regulating ID1, and also demonstrated that the sensitivity of KYSE-150 and TE-1 to the chemotherapeutic drug cisplatin, which is one of the most commonly used chemotherapeutic drugs for treating esophageal cancer, was increased after knockdown of TCF3 or ID1 by siRNA. Here, ID1 is linked to esophageal cancer.