We performed an RNA-seq assay on ESCC cells after the knockdown of TCF3 and found that it may regulate ID1 after relevant measurements (Figure 3A), so we determined the mRNA and protein expression levels of ID1 after TCF3 was knocked down, and the results suggested that the expression level of both ID1 protein and mRNA decreased subsequently with the knockdown of TCF3 in TE-1 and KYSE-150 cell lines (Figure 3B–E). The gene discussed is ID1; the disease is esophageal squamous cell carcinoma.