NGF and cancer: Interestingly, conditioned medi from cultured primary cancer cells from TPAC mice augmented neurite outgrowth similarly to positive control media supplemented with 10 ng/mL nerve growth factor (NGF) (TPAC: 1.822; SFM: 1.05 neurites per 2,500 μm2) (Figure 1, F and G), whereas the number of neurites in cultures with conditioned media from the remaining genotypes did not differ from negative control (KPC: 1.235, TPC: 1.411 neurites per 2,500 μm2) (Figure 1, F and G).