Single gene analysis of ssGSEA was performed, and the results showed glycosphingolipid biosynthesis, phenylalanine metabolism, and PPAR signaling pathway were enriched in B4GALT5 high expression samples, while allograft rejection, autoimmune thyroid disease, graft–versus–host disease, intestinal immune network and primary immunodeficiency were enriched in B4GALT5 low expression samples (Figure 6A). Here, B4GALT5 is linked to autoimmune thyroid disease.