LYVE1 and breast carcinoma: Moreover, in further studies with the mouse 4T1 mammary carcinoma line that can spread directly from the first draining lymph nodes to the blood circulation in Balb/c animals by invading HEVs [51, 52] we found the initial invasion of local lymphatics and metastasis to draining inguinal lymph nodes is both reliant on HA and LYVE-1 and severely impaired in Lyve1−/−mice (Johnson LA and Jackson DG unpublished).