TNF and tuberculosis: This work presents an accurate selection process startingfrompreviously validated T cell epitopes and finalized using a recognizedmethod like the ex vivo antigen-recall assay on Mtb-sensitized PBMCs.61 Our resultspointed out that the peptide pool PM1 successfully stimulatedthe production of IFNγ and TNFα, pivotal cytokines inthe control of TB, and supported the published evidence on the immunogenicityof the contained single peptides.11,15