NFKB1, TLR9, and HDAC4 were predicted to be upregulated in glaucoma and their mRNA level increased according to the RT-qPCR data (Fig. 7), meanwhile, IL18 was predicted to be downregulated in glaucoma and its mRNA level was also increased according to RT-qPCR. This evidence concerns the gene TLR9 and glaucoma.