The first step was to confirm that ApoE−/− mice gained body weight, showed increased visceral and subcutaneous adiposity in addition to hypertriglyceridemia and hypercholesterolemia (Figure S2A–C) in comparison with Control STD, being significantly higher in ApoE−/− mice fed with HFD for 18 weeks. Here, APOE is linked to familial hypercholesterolemia.