In human differentiated THP-1 cells (a human leukemia monocytic cell line), knockdown of PGAM5 stimulated TNFα and IL-6 secretion in naïve macropahges and increased TNFα, IL-6 and IL-1β in macropahges treated with IL-4 and IL-13 (M2 macrophage). This evidence concerns the gene TNF and leukemia.