Hence, due to the importance of the issue, the present study was conducted to assess the effect of maternal PCOS on the first trimester (integrated :NT, PAPP-A, and free-β-hCG) and second trimester (quad marker :HCG, AFP, UE3, and inhibin-A) screening of aneuploidy. The gene discussed is PAPPA; the disease is polycystic ovary syndrome.