Logistic regression model outcome revealed that the screening parameters of free- β-HCG (OR = 1.020, 95% CI: 1.001–1.040, P = 0.040), inhibin-A (OR = 1.049, 95% CI: 1.016–1.082, P = 0.003) and AFP (OR = 1.32, 95% CI: 1.009–1.055, P = 0.007) were related to PCOS. The gene discussed is AFP; the disease is polycystic ovary syndrome.