Therefore, we first investigated whether the colon cancer cell line HCT116 and the hepatocellular cancer cell line Huh7 were able to express and to conjugate both endogenous FAT10 upon treatment with IFNγ and TNF (Fig S1A) and overexpressed FLAG-tagged FAT10 (Fig S1B). The gene discussed is TNF; the disease is malignant colon neoplasm.