CD28 and glioblastoma: To investigate the key effect made by various cytokines produced by macrophages in TME, we visualized the contribution of different types of it in heat map form, and the results showed that: immune inhibitor factors such as TGFBR1 and IL10; MHC molecules such as B2M and HLA-A; immunostimulatory factors such as CD28 and CD48; lymphokines such as Act CD8 and Act CD4 had a positive correlation with RP2 in both GBM and LGG (Figure 10A–10D), Noteworthily, it is conformed that Macrophages may promote the progression of glioma through secrete cytokines like IL10 and TGF-β.