At the single cell level, cell communication analysis also suggested that the signaling pathways such as WNT, VEGF, and TGF-β, which are widely known associated with tumor malignancy, were up-regulated in the high SGS group [17–19] NOTCH signaling has also been reported to be associated with the maintenance of stemness in breast cancer stem cells [20]. This evidence concerns the gene TGFB1 and breast cancer.