were also able to demonstrate that ASS1 deficiency in cancer increases cytosolic aspartate levels leading to increased activation of the enzymatic complex CAD (carbamoyl-phosphate synthase 2, aspartate transcarbamylase, dihydroorotase complex) by upregulating its substrate availability and by increasing its phosphorylation by S6K1 through the mTOR pathway. This evidence concerns the gene MTOR and cancer.