As shown in Figure 3, there was a significant decrease in plasma levels of C18SO, C18SA, C18SAdiene C16SO, and C17SO but not of C20SO in Abca1 HSKO mice Because plasma triglyceride concentrations are elevated in Tangier disease subjects (Clee et al., 2000; Kolovou et al., 2003) and non-fasted Abca1 HSKO mice (Chung et al., 2010), we performed a similar sphingoid base profiling experiment with non-fasted Abca1 HSKO mouse plasma, which gave similar results (Supplementary Figure S1). This evidence concerns the gene ABCA1 and Tangier disease.