For the first-line RCTs, our study showed that clinico-pathological characteristics of patients which correlated with preferable OS were no prior local therapy, EHS, AFP greater than or less than 400 ng/ml, viral hepatitis positivity, ECOG 1, MVI, MVI and/or EHS, BCLC C disease, age greater than or less than 65 years, and male. This evidence concerns the gene AFP and viral hepatitis.