HIF1A and age-related macular degeneration: Here, we used a small Cas9 ortholog derived from Neisseria meningitidis (Nme2Cas9) that could be packaged with sgRNA into a single AAV,21 and designed four sgRNAs targeting the relatively conservative functional domains of Hif1α, Vegfa and Vegfr2 genes, to effectively silence wet AMD‐associate genes (Figure 1A).