Here, we used a small Cas9 ortholog derived from Neisseria meningitidis (Nme2Cas9) that could be packaged with sgRNA into a single AAV,21 and designed four sgRNAs targeting the relatively conservative functional domains of Hif1α, Vegfa and Vegfr2 genes, to effectively silence wet AMD‐associate genes (Figure 1A). This evidence concerns the gene KDR and age-related macular degeneration.