GSDMD and infection: In NLRC4 KO, NLRP3 KO (Figures 3F and 3G), and WT THP-1 cells (Figure 5D), GSDMD cleavage occurred even when the axoU gene was disrupted, while in the NLRC4/NLRP3 KO THP-1 no pyroptosis occurred upon infection with wildtype or AC055pGPI-axoU (Figure 5D).