Next, we further observed that the TLS signature expression was upregulated in the estrogen receptor (ER)-negative group, progesterone receptor (PR)-negative group, HER2-negative group, and triple-negative breast cancer (TNBC) subtype in the METABRIC dataset, while the result showed no statistically significant in HER2 group (Fig. S4A–D). The gene discussed is PGR; the disease is triple-negative breast carcinoma.