RREB1 and posterior cortical atrophy: qRT‒PCR and western blot analysis were performed in let-7a-overexpressing or let-7a-knockdown PCa cells, and the results suggested that RREB1 expression was significantly upregulated in response to let-7a knockdown, whereas let-7a overexpression dramatically decreased the expression level of RREB1 (Fig. 9l and m).