To capture specific subtypes of breast tumor samples from the TCGA data, we trained GTM-decon on an scRNA-seq reference data from 26 primary tumors of breast cancer (BRCA) patients with three major clinical subtypes of BRCA, including 11 ER + , 5 HER2 + and 10 TNBC [44]. The gene discussed is ESR1; the disease is breast carcinoma.