Therefore, we further evaluated the infiltration of M1 and M2 types of macrophages in the tumor area, and the results showed that CD86 expression was significantly increased and CD206 expression was decreased in the tumor area after PCMT1 knockdown, which may indicate that PCMT1 affects the polarization of macrophages in the tumor area, these evidences all suggest that PCMT1 may affect the immune microenvironment of tumors, and inhibition of PCMT1 may better promote anti-tumor immunity (Additional file 3). Here, CD86 is linked to neoplasm.