PPP3CA and Parkinson disease: To further investigate the role of CnA and the possible involvement of the NFAT transcription factor pathway in these cellular models of PD, we differentiated N2a cells into dopaminergic phenotype by treating the cells with butyryl AMPc in reduced serum medium for 72 h, as previously described (Tremblay et al. 2010; Wu et al. 1998).