In summary, our data shows that aSyn-O exogenously given to neurons leads to the activation of CaN/NFAT pathway that is sufficient and necessary to induce a neurodegeneration phenotype like PD, as neuron cells death and loss of functional synapses as shown by the loss of neuron ramification and synapsin-1 punctas. The gene discussed is NUP214; the disease is Parkinson disease.