Regarding the ten genes with a significant differential mutation frequency between the two signatures, mutation of EP400[38], RNF43[39], TRPM3[40],TEP1[41], MCF2[42] have been reported to associated with pathogenesis or prognosis of CRC, and the expression of DSCAML1[43], RYR1[44] and DNAH5[45] was related to CRC development. Here, RYR1 is linked to colorectal carcinoma.