ACTA1 and Hepatic fibrosis: We quantified stains of the liver fibrosis markers, low-affinity nerve growth factor receptor (NGFR)19,20 and alpha-smooth muscle actin (ASMA)21, which revealed the zonation of the capsule, such that expression of both NGFR and ASMA increased from the metastasis edge towards the rim-liver interface (Fig. 3a–c).