These immune checkpoints were positively correlated with immune-promoting genes, such as CD86 and CD80 (Fig 5A and 5B, p<0.05), and significantly negatively regulated, indicating that MLK4 may affect the invasion of immune cells in cervical cancer by regulating the expression of immune checkpoints and immune-related genes, leading to immune escape of tumors and affecting the survival of patients. This evidence concerns the gene CD86 and cervical cancer.