To examine if LARP4 is enriched in viral factories during vD9muD10mu infection, we used the A549DKO cell line that allows VACV replication by knocking out two antiviral genes encoding PKR and RNase L. The knockout of RNase L also ensures intact cellular and viral mRNAs in vD9muD10mu infection, as VACV could activate RNase L by viral dsRNA for RNA cleavage. Here, RNASEL is linked to infection.