MKI67 and pancreatic neoplasm: To confirm the anti-proliferative effect of ASM and ASU on a molecular level, protein lysates from Capan-2-treated cells were immunoblotted with an anti-Ki67 antibody, which is a widely used biomarker that reflects the cell proliferation state and indicates the prognosis for many cancers, including pancreatic cancer (Lee et al., 2013).