Mutant fraction was highest in HCC patients with a mean value of 1.81% for TP53 c.746G>T p. R249M, 0.95% for TP53 c.747GT p. R249S, and 1.73% for CTNNB1 c.133T>C S45P (Table 4). The gene discussed is TP53; the disease is hepatocellular carcinoma.