Detection of TP53 gene c.746G>T (p.R249M), c.747G>T (p.R249S), and CTNNB1 gene c.133T>C (p.S45P) mutations in healthy, CHB, and HCC patients was carried out by a dual-probe based ddPCR assay, which can detect both wild-type and mutant types (Figure 2). The gene discussed is TP53; the disease is hepatocellular carcinoma.