In addition to these analyses, to delineate potential factors impeding the infiltration of CXCR6+ T cells and potential differences of CXCR6+ and CXCR6- subsets, we analyzed the correlations of tumor infiltrating M2 type macrophages and cell subsets and found that CD8+CXCR6+GZMK+PD1+ T cells were negatively correlated with M2 type macrophages, whereas CD4+CXCR6-GZMK-PD1- cells were positively correlated with M2 type macrophages (Figure 10N, Additional file 1: Figures S10G–I). The gene discussed is PDCD1; the disease is neoplasm.