In genomes from adenocarcinoma of the colorectum (n = 7) and uterus (n = 1), 11-mers with 5 + hotspots assigned to the mutational signature 62 of POLE deficiency displays mutation rates 4-fold higher (2,361 SNV/Mb/patient; 3 Mb) than all signature 62-assigned 11-mers (585 SNV/Mb/patient; 208 Mb; p < 10–318; binomial test; Fig. 5f and Additional file 1: Fig. S2). The gene discussed is POLE; the disease is adenocarcinoma.