To assess the progression of diabetes in HFD/STZ-MICU1ecKO mice at the ages of 18 weeks, the intraperitoneal glucose tolerance tests (IPGTTs) and insulin tolerance tests (ITTs) were performed as shown in Fig. S2, and the results showed that HFD/STZ-MICU1ecKO mice had higher blood glucose level and lower clearance efficiency of plasma glucose than HFD/STZ-WT mice (Fig. S2). Here, INS is linked to diabetes mellitus.