In general, nodes with mutations in signaling genes (NRAS, KRAS, JAK2, FLT3, PTPN11) were located at the very end of each branch, representing last events in driving leukemia, which was also confirmed by a separate analysis using Bradley-Terry models (Supplementary Fig. S4) [19, 22, 29] The DNMT3A node produced two other branches with IDH1 and IDH2R172 departing separately from the DNMT3A node suggesting mutual exclusivity. This evidence concerns the gene FLT3 and leukemia.