Several in vitro functional rescue experiments were performed in TRIM26-upregulated or TRIM26-silenced osteosarcoma cells after overexpressing or silencing RACK1, and the results showed that the decreased malignant behaviors including cell proliferation and invasion in TRIM26-upregulated cells were reversed when RACK1 was overexpressed, whereas RACK1 knockdown diminished the increased malignant phenotypes in TRIM26-silenced osteosarcoma cells (Supplementary Fig. 5A–F). This evidence concerns the gene RACK1 and osteosarcoma.