Moreover, our single-cell analysis showing altered microenvironments based on tumor genotype is consistent with recent melanoma brain metastasis single-cell landscaping analysis showing differential lymphocyte populations in intracranial lesions.14 Given that our patient cohort who underwent snRNA-seq was treatment naive, this altered microenvironment appears to be an intrinsic property of BRAF V600E variant melanoma brain metastases, although systemic therapies likely also play an important role in modulating the microenvironment. The gene discussed is BRAF; the disease is melanoma.