2-ME2 was found to induce nNOS in not only cancerous cell lines such as osteosarcoma 143B [9, 10, 14], and MG63.2 [73], glioblastoma SW1088 [31], but also in mouse hippocampal HT22 cells [9], leading to the elevation of NO and a resultant cell death [9]. Here, NOS1 is linked to osteosarcoma.