We expressed a fusion protein of GtCCR4-3.0-EYFP, which contains the membrane-trafficking signal and the endoplasmic reticulum (ER)-export signal from a Kir2.1 potassium channel (Gradinaru et al., 2010; Hoque et al., 2016), or Myc-tagged GtCCR4 (GtCCR4-MT) and TagCFP separately in neuronal ND7/23 cells, which are a hybrid cell line derived from rat neonatal dorsal root ganglia neurons fused with mouse neuroblastoma (Wood et al., 1990). Here, MCAT is linked to neuroblastoma.