First, the GPR gene signature was a risk factor for overall survival of BLCA patients and negatively correlated with the immune response of BLCA patients, while the TME signatures was a protective factor for overall survival of BLCA patients and positively correlated with the immune response of BLCA patients, and the interaction between the two signatures within each other needs to be further investigated. Here, ALDH18A1 is linked to bladder transitional cell carcinoma.