Trajectory analysis showed consistent differentiation trends between the C11 and C2 clusters in fibroblasts, but C2 was not a tumorigenesis-associated cell cluster, and the distinction was that the specifically characterized genes were different, whereas the mechanism of HSPB6 in PAAD was unknown and its function in fibroblast was unclear. Here, HSPB6 is linked to pancreatic adenocarcinoma.