SMPD3 and metabolic dysfunction-associated steatohepatitis: The resultant DNA fragments were detected using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and EpiTYPER (Agena Bioscience, San Diego, CA, USA) was employed to quantify the CpG methylation level of Smpd3 in liver and adipose of NASH and mild fibrosis mice.